Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 2
Release date: Apr 5 2014
Last update date: Apr 5 2014
Access: Public
Chemicals: Phosphates
Dataset link Phosphate Starvation in Chlamydomonas reinhardtii

Experimental Protocol


Chlamydomonas reinhardtii strain 21 gr (CC1690, wild-type) grown in TAP medium (Harris 1989) in a rotary incubator (200 rpm) at 25 °C in continuous light (70 µmol m-2 s-1). For 24 hours, either 1.1 mM phosphate or 0 mM were provided with the growth media. P deprivation was achieved by washing cells twice in midlogarithmic growth phase with liquid TAP medium without P (TAP-P) and cells were resuspended at a density of 2.5 mg/ml Chlorophyll in TAP or TAP-P. Cell aliquots were collected for mRNA isolation 24 h after being transferred either to TAP or TAP-P medium.

Repositories


GEO

GSE56505

ArrayExpress

E-GEOD-56505

ENA

SRP040944

BioProject

PRJNA243546

Download


Contact


Arthur Grossman