|Application:||Gene expression microarray analysis|
|Number of samples:||2|
|Release date:||Sep 30 2013|
|Last update date:||May 11 2017|
|Diseases:||Bone Marrow Diseases, Deficiency Diseases|
|Dataset link||Expression analysis from Runx2-deficient pDCs from mouse|
Total BM cells were isolated from Runx2-/- and wildtype fetal liver chimeras 2 months post-reconstitution. BM cells were stained with antibodies against surface markers CD11b, CD11c, BST2, and B220. CD11b- BST2+ B220+ pDCs were purified by flow cytometry on a BD FACS Aria. RNA was purified immediately and prepared for microarray analysis using the Ambion WT labeling kit. Expression was analyzed using Affymetrix Mouse Gene 1.0 ST.