Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Sep 30 2013
Last update date: May 11 2017
Access: Public
Diseases: Bone Marrow Diseases, Deficiency Diseases
Dataset link Expression analysis from Runx2-deficient pDCs from mouse

Experimental Protocol


Total BM cells were isolated from Runx2-/- and wildtype fetal liver chimeras 2 months post-reconstitution. BM cells were stained with antibodies against surface markers CD11b, CD11c, BST2, and B220. CD11b- BST2+ B220+ pDCs were purified by flow cytometry on a BD FACS Aria. RNA was purified immediately and prepared for microarray analysis using the Ambion WT labeling kit. Expression was analyzed using Affymetrix Mouse Gene 1.0 ST.

Repositories


GEO

GSE49859

ArrayExpress

E-GEOD-49859

BioProject

PRJNA215105

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Contact


Catherine Sawai

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