Dataset features

Specifications


Application: ChIP-seq analysis, Flow cytometry
Number of samples: 40
Release date: Apr 24 2018
Last update date: Oct 23 2018
Access: Public
Dataset link Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere sequestration

Experimental Protocol


DNA replication timing was studied using leading strand polymerase Chromatin Immunoprecipitation seq (ChIP-seq) in cells released from an alpha factor block (G1 block) at different timepoints and quantitative sequencing of asynchronous FACS sorted S-phase cells (sort-seq). Protein binding of Rif1 was evaluated by Chromatin endonuclease cleavage seq (ChEC-seq)

Repositories


GEO

GSE97953

ENA

SRP104236

BioProject

PRJNA383476

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Contact


Lukas Hafner