Computational protocol: Preventing carbon nanoparticle-induced lung inflammation reduces antigen-specific sensitization and subsequent allergic reactions in a mouse model

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Protocol publication

[…] Flow cytometry was performed with a FACScanto II Flow Cytometer (BD Bioscience, BD Bioscience, Franklyn Lake, NJ, USA) and analysed with FlowJo 7.6.5. Fluorescently labelled CD11c (N418), GR-1 (RB6-8C5), MHCII (M5/144.15.2), CD4 (GK1.5), CD8b (H35-17.2), CD19 (MB19-1), CCR7 (4B12), F4/80 (BM8) and biotin anti-APC (APC003) were used in various combinations. Antibodies used were from BioLegend (San Diego, CA, USA), except for CD19 (eBioscience, San Diego, CA, USA), CD4 and CD8 (BD Pharmingen). Dendritic cells and macrophages loaded with OVA-488 were also detected by using flow cytometry. [...] Statistical calculations were performed using IBM SPSS statistics 22. Significant values were calculated either by ANOVA analyses with Tukey’s HSD post hoc testing or by comparison of individual groups by Mann–Whitney U-test. Mean values with standard errors are given. Power calculations for the design of animal experiments were performed using G*Power version 3.1.5 (University of Kiel, Germany). Graphs display means and standard errors. […]

Pipeline specifications

Software tools FlowJo, SPSS, G*Power
Applications Miscellaneous, Flow cytometry
Organisms Mus musculus
Diseases Drug Hypersensitivity, Immune System Diseases, Pneumonia
Chemicals Carbon