Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Jun 12 2018
Last update date: Jun 12 2018
Access: Public
Diseases: Heart Diseases
Dataset link RNA-Seq analysis of hearts from NBCe1 Na-HCO3 cotransporter cardiac conditional knockout (K) vs wild-type (W) mice

Experimental Protocol


An NBCe1 (Slc4a4 gene) conditional knockout mouse model was prepared. For cardiac myocyte-specific deletion of Slc4a4 exon 12, the mice were bred with a C57Bl6 mouse carrying the Cre recombinase gene driven by the β-myosin promoter, which is active in cardiac myocytes. Total RNA was isolated from whole hearts of 4-month-old male Slc4a4flx/flx and Slc4a4flx/flx(Cre) mice (n = 6 of each genotype). RNA Seq analysis was performed using the Illumina HiSeq 1000 platform, and sequence reads were aligned to the reference mouse genome using TopHat aligner. Statistical analysis was performed using the negative-binomial model of read counts. mRNA expression data are presented as Reads Per Kilobase per Million mapped reads.

Repositories


GEO

GSE115579

ENA

SRP150211

BioProject

PRJNA475532

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