Dataset features


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Aug 13 2015
Last update date: Aug 16 2018
Access: Public
Dataset link Dose-Responsive Gene Expression in Suberoylanilide Hydroxamic Acid (SAHA) Treated Resting CD4+ T Cells

Experimental Protocol

Primary CD4+ T cells were isolated from human peripheral blood drawn from 6 healthy HIV-seronegative donors. For each donor sample, CD4+ T cells were counted and aliquoted into 6-well plates; 5 million cells per well at a concentration of 2.5 million cells per mL of media (RPMI 1640 with 5% human serum AB). Wells containing CD4+ T cells were treated with SAHA (Merck) at 0.34 μM, 1 μM, 3 μM, or 10 μM in 0.1% DMSO, or with only 0.1% DMSO (vehicle control). Treated cells were incubated at 37°C, 5% CO2 for 24 hours. Treated cells were then collected for microarray analysis of mRNA in SAHA treated cells versus DMSO treated cells.