Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Sep 6 2013
Last update date: Aug 13 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Chemicals: Amino Acids, Estrogens
Dataset link A Common Docking (CD) Domain in Progesterone Receptor-B Links MKP3-Dependent Rapid Signaling Events to JAK/STAT Regulation of Gene Expression Required for Breast Cancer Cell Proliferation

Experimental Protocol


The study contains 6 different sample groups measured in triplicate, for a total of 18 individual samples (18 arrays). From parental T47D-Y human breast cancer cell lines, we created three stable clones expressing (1) an empty vector (pSG5), (2) the wild type progesterone receptor isoform B (pSG5-PR-B), or (3) a mutant mutant CD domain PR-B. These cell lines were treated with either (1) vehicle control (ethanol) or (2) R5020 10e-8 M for 6 hours before total RNA harvest. Thus, the experiment contains three cell lines, and two treatments (6 sample groups) treated and analyzed in triplicate (18 microarrays). Standard Illumina HT-12v4 chip controls were used during hybridization.

Repositories


GEO

GSE46850

ArrayExpress

E-GEOD-46850

BioProject

PRJNA202844

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Contact


Todd Knutson
Todd P Knutson

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