Computational protocol: Hmgcr in the Corpus Allatum Controls Sexual Dimorphism of Locomotor Activity and Body Size via the Insulin Pathway in Drosophila

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Protocol publication

[…] A previously described paradigm was used , . Two, four, or five days old flies were allowed to walk in a small square arena (4×4 cm, 3.5 mm high) for five hours. A camera placed above the arena recorded fly movements (Ethovision, Noldus, Netherland). The number of activity and inactivity phases (also equivalent to the starts/stops number) was quantified by the computer software. Since in some cases, the level of locomotor activity differs among groups with different genotypes, the number of start/stop was normalized by designating the females of each group as the reference (expressed as 100%) and comparing males in the same group to the females. Flies and their respective controls were always recorded at the same time. All experiments were performed at 24°C and 60% humidity. Statistical comparisons were made with ANOVA tests, using Statistica software (StatSoft, Inc.). […]

Pipeline specifications

Software tools EthoVision, Statistica
Applications Miscellaneous, Macroscope & basic digital camera imaging
Organisms Drosophila melanogaster
Diseases Dwarfism, Pituitary
Chemicals Cholesterol