|Application:||Gene expression microarray analysis|
|Number of samples:||115|
|Release date:||Feb 23 2018|
|Last update date:||Aug 13 2018|
|Dataset link||Transcriptomic profiling of rhesus macaques vaccinated with Ad26/ALVAC+gp120 or DNA/ALVAC+gp120|
Twenty four (24) rhesus macaques were randomized to two vaccination groups. One group (n=12) was primed once with Ad26 (at week 0) and boosted twice with ALVAC-SIV/gp120 in Alum adjuvant (at week 12 and week 24). The second group (n=12) was primed twice with DNA (at week 0 and week 4) and boosted twice with ALVAC-SIV/gp120 in Alum adjuvant (at week 12 and week 24). Blood samples were taken pre-vaccination, 24 hours after the first boost (week 12), 2 weeks after the first boost (week 14), 24 hours after the second boost (week 24) and 1 week after the second boost (week 15). All the samples were taken before SIV challenge. One animal (R270) who received the Ad26/ALVAC+gp120 vaccine was not included in the transcriptomic study because of a lack of blood samples. Blood samples were conserved in PAXgene tubes. RNA was extracted and hybridized to Illumina beadchips.