Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 115
Release date: Feb 23 2018
Last update date: Aug 13 2018
Access: Public
Dataset link Transcriptomic profiling of rhesus macaques vaccinated with Ad26/ALVAC+gp120 or DNA/ALVAC+gp120

Experimental Protocol


Twenty four (24) rhesus macaques were randomized to two vaccination groups. One group (n=12) was primed once with Ad26 (at week 0) and boosted twice with ALVAC-SIV/gp120 in Alum adjuvant (at week 12 and week 24). The second group (n=12) was primed twice with DNA (at week 0 and week 4) and boosted twice with ALVAC-SIV/gp120 in Alum adjuvant (at week 12 and week 24). Blood samples were taken pre-vaccination, 24 hours after the first boost (week 12), 2 weeks after the first boost (week 14), 24 hours after the second boost (week 24) and 1 week after the second boost (week 15). All the samples were taken before SIV challenge. One animal (R270) who received the Ad26/ALVAC+gp120 vaccine was not included in the transcriptomic study because of a lack of blood samples. Blood samples were conserved in PAXgene tubes. RNA was extracted and hybridized to Illumina beadchips.

Repositories


GEO

GSE108011

BioProject

PRJNA422217

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Contact


Slim Fourati