Dataset features


Application: ChIP-on-chip analysis
Number of samples: 238
Release date: Jan 1 2013
Last update date: Mar 21 2017
Access: Public
Diseases: Breast Neoplasms
Dataset link ChIP-chip of 38 nuclear receptors and NR co-factors in breast cancer cell line MCF7

Experimental Protocol

Input DNA was used as control against all 38 ChIPchip samples. All samples are done in triplicates. We mapped the binding sites in the bacterial artificial chromosome (BAC) transgenic MCF7 cells in which we tagged the transcription factors with a modified LAP (localization and affinity purification) tag containing green fluorescent protein (GFP). Goat anti-GFP (raised against His-tagged full-length eGFP and affinity-purified with GST-tagged full-length eGFP) was used to perform ChIP experiments in those transgenic lines. *Bed files for binding sites of each of 38 factors (TR2L and TR2S are two isoforms of TR2) were provided as additional results files. Please note that the folllowing data files are re-processed from Dr. Sujun Hua's GSE10800 and GSE15244 uploads. ESR1.bed FOXA1.bed GATA3.bed RARA.bed RARG.bed Since the authors have updated the analysis pipeline and presented the changed results in the associated publication, these updated results are included in the records (Dr. Sujun is one of the contributors in this work).








Jie Zhou