Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 23
Release date: Feb 27 2018
Last update date: Sep 21 2018
Access: Public
Genes: AGO2
Taxon: Drosophila melanogaster
Dataset link Argonaute2 and LaminB modulate gene expression by controlling chromatin topology (neuRNA-seq)

Experimental Protocol


Nascent RNA-seq (neuRNA-seq) in mock, AGO2, LaminB, and Dcr-2 knockdowns. In addition, AGO2 was depleted using an alternative siRNA designed against its 5'UTR (si5UTRAGO2) and rescue with a wild type version of AGO2 (pwahf_ago2) or catalytic mutant AGO2V966M (pwahf_v966m). Three separate experiments are included here, and each experiment has between 2 and 3 biological replicates as indicated. The supplemental counts table contains raw read counts in genes in each sample, and the supplemental_sample_lookup table describes the experimental design. The "experiment" column of the supplemental_sample_lookup table indicates the experiment from which each sample came from. When performing differential expression analysis with these counts, only samples that came from the same experiment should be compared; in particular note that each experiment has its own set of mock control samples.

Repositories


GEO

GSE95845

ENA

SRP101589

BioProject

PRJNA378550

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Contact


Ryan Dale

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