Computational protocol: Up-regulation of A20/ABIN1 contributes to inefficient M1 macrophage polarization during Hepatitis C virus infection

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Protocol publication

[…] Cells were fixed with 2 % paraformaldehyde and permeable reagent (eBioscience) for 30 min on ice. Cells were stained with fluorescently labeled antibodies (1 μg/ml; 1 h at room temperature), assayed by flow cytometry (FASCAria II, BD Bioscience, Franklin Lakes, NJ, USA), and data were analyzed by Flowjo7.6 software (Tree Star, Inc. Ashland, OR, USA). Primary antibodies used for staining were anti-human cd71-FITC (eBioscience), mAb to HCV core Antigen (Abcam), and goat anti-mouse Alexa555 antibody (Abcam). [...] One-way ANOVA and Student’s t-test were used to determine statistical significance. Analyses were performed with GraphPad Prism Version 5.0 (La Jolla, CA, USA) and SPSS Statistics 21.0 (IBM Corp., Armonk, NY, USA). A p value < 0.05 was considered statistically significant. […]

Pipeline specifications

Software tools FlowJo, SPSS
Applications Miscellaneous, Flow cytometry
Organisms Homo sapiens
Diseases Hepatitis C, Neoplasms
Chemicals Alanine