Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 1
Release date: Sep 23 2016
Last update date: Nov 21 2018
Access: Public
Dataset link Aire-KO MEChi RNAseq profiling

Experimental Protocol


~100ng of total RNA was isolated by Trizol extraction from MHC-II high mTECs from a pool of 3 Aire-KO mice. Poly-A-selected transcriptome libraries were generated using the non-directionnal TruSeq V3 RNA Sample Prep Kit (without additional pre-amplification) following the manufacturer's protocols. Enrichment of DNA fragment with adapter molecules on both ends was done using 15 cycles of PCR amplification using the Illumina PCR mix and primer cocktail. Paired-end (2 × 100 bp) sequencing was performed using the Illumina HiSeq2000 machine.

Repositories


GEO

GSE87133

ENA

SRP090189

BioProject

PRJNA343647

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Contact


Matthieu Giraud

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