Cas-Designer statistics

Tool stats & trends

Looking to identify usage trends or leading experts?

Protocols

Cas-Designer specifications

Information


Unique identifier OMICS_09915
Name Cas-Designer
Software type Package/Module
Interface Command line interface
Restrictions to use None
Output data Cas-Designer shows the results in an interactive table. Output data can be filtered according to GC content, out-of-frame scores, or mismatch numbers.
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline Cas-Designer

Information


Unique identifier OMICS_09915
Name Cas-Designer
Interface Web user interface
Restrictions to use None
Output data Cas-Designer shows the results in an interactive table. Output data can be filtered according to GC content, out-of-frame scores, or mismatch numbers.
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Cas-Designer

Publications for Cas-Designer

Cas-Designer citations

 (10)
library_books

CRISPR/Cas9 mediated gene knockout of Anopheles gambiae FREP1 suppresses malaria parasite infection

2018
PLoS Pathog
PMCID: 5843335
PMID: 29518156
DOI: 10.1371/journal.ppat.1006898

[…] ng any nucleotide. We checked candidate gRNA sequences for potential off-target binding using the following web tools: http://zifit.partners.org/ZiFiT/, http://crispr.mit.edu/, http://www.rgenome.net/cas-designer/, and http://chopchop.cbu.uib.no/, and retained three 20-nucleotide specific target sequences that were followed by a PAM and had the fewest predicted genomic off-targets. The final selec […]

library_books

Engineering cell signaling using tunable CRISPR–Cpf1 based transcription factors

2017
Nat Commun
PMCID: 5727435
PMID: 29235474
DOI: 10.1038/s41467-017-02265-x

[…] sembled by fusing dAsCpf1(D908A) with VP64 and VPR, respectively, at the C terminus. The plasmid maps are shown in Supplementary Fig. . The original crRNAs were designed using the online design tool “Cas-Designer” (http://www.rgenome.net/cas-designer/). The designed complimentary DNA (cDNA) sequence for each crRNA was synthesized and inserted into the corresponding plasmid expressing both dAsCpf1 […]

call_split

ID3 regulates the MDC1 mediated DNA damage response in order to maintain genome stability

2017
Nat Commun
PMCID: 5638908
PMID: 29026069
DOI: 10.1038/s41467-017-01051-z
call_split See protocol

[…] The ID3 CRISPR (Guide sequence insert: 5ʹ-CGAGGCGGTGTGCTGCCTGTCGG)-3ʹ construct targeting exon 1 of the human ID3 gene were designed using the Cas9-Designer (http://www.rgenome.net/cas-designer). SpCas9 expression plasmids (500 ng) and sgRNA plasmids (500 ng) were transfected to 2 × 105 U2OS cells using 4D-nucleofector (Lonza) SE kit and program CM-104 in 20 µl Nucleovette Strip […]

library_books

ARNT is a potential direct HIF 1 target gene in human Hep3B hepatocellular carcinoma cells

2017
PMCID: 5571568
PMID: 28855849
DOI: 10.1186/s12935-017-0446-2

[…] employed. Due to the high enrichment of both transcription factors in Region 3 appropriate CRISPR/Cas9 targets within this sequence were investigated using a web based tool [] (http://www.rgenome.net/cas-designer/). Therefore, two different CRISPR/Cas9 target sites were selected. The sequence designated as Target 1 covers the HBS upstream of the TATA box but might be error prone due to numerous si […]

library_books

CRISPR/Cas9 mediated gene knockout screens and target identification via whole genome sequencing uncover host genes required for picornavirus infection

2017
PMCID: 5481571
PMID: 28446605
DOI: 10.1074/jbc.M117.782425

[…] ,840 pairs of oligonucleotides that encoded sgRNAs, collectively targeting 10,280 protein-coding genes, were individually synthesized and cloned in an sgRNA expression plasmid. Using Cas-OFFinder and Cas-Designer (available at http://www.rgenome.net), we carefully chose three target sites per gene to avoid off-target effects and in-frame mutations as much as possible (, ). All sgRNAs in the librar […]

library_books

Heritable Genomic Fragment Deletions and Small Indels in the Putative ENGase Gene Induced by CRISPR/Cas9 in Barley

2017
Front Plant Sci
PMCID: 5404177
PMID: 28487703
DOI: 10.3389/fpls.2017.00540

[…] manually according to certain criteria (guanine was selected as transcriptional start site and high purine content of the 6 nt adjacent to the PAM was favored) in the case of t3 and tB (; ), or using Cas-Designer (http://www.rgenome.net/cas-designer/) in the case of t1, t2, and tA. Each sgRNA targeted a different site in the putative barley ENGase gene, and only t1 was located on the sense strand. […]

Citations

Looking to check out a full list of citations?

Cas-Designer institution(s)
Department of Chemistry, Hanyang University, Seoul, Korea; Institute for Materials Design, Hanyang University, Seoul, Korea; Center for Genome Engineering, Institute for Basic Science, Seoul, Korea; Department of Chemistry, Seoul National University, Seoul, Korea

Cas-Designer reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review Cas-Designer