Cas-OFFinder statistics

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Cas-OFFinder specifications

Information


Unique identifier OMICS_04720
Name Cas-OFFinder
Software type Package/Module
Interface Command line interface
Restrictions to use None
Input data gRNA seq (batches)
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

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Information


Unique identifier OMICS_04720
Name Cas-OFFinder
Interface Web user interface
Restrictions to use None
Input data gRNA seq (batches)
Computer skills Basic
Stability Stable
Maintained Yes

Publication for Cas-OFFinder

Cas-OFFinder in publications

 (62)
PMCID: 5945874
PMID: 29748595
DOI: 10.1038/s41467-018-04175-y

[…] in the mouse genome, which differed from the vegfa or hif1a on-target site by up to four nucleotides in aav-cpf1-vegfa or -hif1a treated retina and rpe. potential sites were identified using the cas-offinder program. no off-target indels were detectably induced in aav-cpf1-vegfa or -hif1a injected mouse retina or rpe cells (supplementary fig. ; supplementary table ). taken together, […]

PMCID: 5920046
PMID: 29700298
DOI: 10.1038/s41467-018-04048-4

[…] identified genes linked to immune response using gene set enrichment analysis software, supplemented by manual inspection. off-target site prediction for the pcsk9-targeting grna was conducted using cas-offinder and matched to potential downstream genes through the bedtools suite “closest” function., minced tissue was lysed in ripa buffer (sigma), and total protein quantified via the bca assay […]

PMCID: 5889534
PMID: 29625575
DOI: 10.1186/s13287-018-0839-8

[…] were used to detect the expression of f9., potential off-target sites that differed from the sgrna sequence by up to five nucleotides in the genome were searched using the web-based program cas-offinder (http://www.rgenome.net/cas-offinder/). after insertion was detected in ipscs, genomic dnas extracted from the parental ipscs and the successful inserted ipscs were subjected to whole […]

PMCID: 5880812
PMID: 29610531
DOI: 10.1038/s41467-018-03760-5

[…] staining solution at rt for 10–15 min. 250 μl of 1× binding buffer was added into the mixture and flow cytometry was used to measure cell apoptosis., potential off-target sites were found by using cas-offinder. the mismatches were no more than 6. the off-target sites were amplified by pcr and sequenced by sanger sequencing., hpscs were electroporated with a plasmid mixture of 4 μg of i-scei […]

PMCID: 5905640
PMID: 29529199
DOI: 10.1093/hmg/ddy069

[…] the ngg sequence at the 3’ end (pam domain). the grnas were chosen to exhibit low off-target activity in genic regions of the human genome, according to crispr rgen online tools (http://rgenome.net/cas-offinder/). the grnas were cloned in an expression vector containing a cas9 nuclease expression cassette and a cd4 reporter [geneart® crispr nuclease (cd4 enrichment) vector kit, life […]


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Cas-OFFinder institution(s)
National Creative Research Initiatives Center for Genome Engineering, Department of Chemistry and Department of Physics and Astronomy, Seoul National University, Seoul, Korea

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