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Cas9 Design specifications


Unique identifier OMICS_06785
Name Cas9 Design
Interface Web user interface
Restrictions to use None
Input data Target region sequence (single and batched)
Computer skills Basic
Stability No
Maintained No


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Publication for Cas9 Design

Cas9 Design citations


Regulation of the cohesin loading factor NIPBL: Role of the lncRNA NIPBL AS1 and identification of a distal enhancer element

PLoS Genet
PMCID: 5754091
PMID: 29261648
DOI: 10.1371/journal.pgen.1007137

[…] The expression vectors for active Cas9 and catalytically inactive Cas9 were obtained from Addgene. The guide RNA were designed using the Cas9 design tool ( [] and inserted in the gRNA Cloning Vector (Addgene #41824) [] following the protocol deposited together with the vector. The sequences and posi […]


Minimizing off Target Mutagenesis Risks Caused by Programmable Nucleases

Int J Mol Sci
PMCID: 4632775
PMID: 26501275
DOI: 10.3390/ijms161024751

[…] ce or in a region defined by an accession number according to the target region size. CRISPR Design [,], selects sgRNA from an input sequence up to 250 bp, while CRISPRdirect [,], ZiFit Targeter [,], Cas9 design [,], E-CRISP [,], and CHOPCHOP [,] can perform searches from much longer target regions.The web tool CRISPRdirect searches not only the entire sgRNA sequence for potential off-target sites […]


Integrative Analysis of CRISPR/Cas9 Target Sites in the Human HBB Gene

Biomed Res Int
PMCID: 4396065
PMID: 25918715
DOI: 10.1155/2015/514709

[…] Firstly, to systemically analyze the CRISPR target sites in the region of interest, all candidate sites complying with the GN19NGG sequence pattern are searched using the web tool Cas9 Design ( []. The candidate sites will be classified and numbered based on their locations in the exons and introns. Basic information such as sequence, genome position […]


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Cas9 Design institution(s)
Biomedical Engineering Department, College of Engineering, Peking University, Beijing, China; Center for Bioinformatics, State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China; National Institute of Biological Sciences, Zhongguancun Life Science Park, Beijing, China; Institute of Computer Science and Technology, Peking University, Beijing, China

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