Computational protocol: Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma

Similar protocols

Protocol publication

[…] To compare the distributions of demographic variables and selected risk factors between patients and controls, the Chi-square test was used. The observed number of cases for each genotype in the study and control group was compared with the expected number according to the Hardy-Weinberg principle, using the χ 2 test. The χ 2 analysis was also used to test the significance of the differences between distributions of genotypes in glaucoma patients and controls. The association between case-control status and each polymorphism, measured by the odds ratio (OR) and its corresponding 95% confidence interval (CI), was estimated using an unconditional multiple logistic regression model. When calculating the probability, Pearson correction was used, and if the expected cell values were less than 5, Fisher's exact test was used. The odds ratios were then adjusted for possible interfering factors, including hypertension, low blood pressure, vascular disease, and diabetes. Clinical features in patients with POAG were also compared between genotypes of each polymorphism by using an analysis of variance (ANOVA with post hoc Tukey's HSD test) for the comparison with discrete variables. The nonparametric Mann-Whitney U test was applied to determine the levels of mRNA expression in blood of POAG patients and healthy subjects. The nonparametrical statistical tests (ANOVA with post hoc Tukey's HSD test) were applied to compare the level of mRNA expression with genotypes of each polymorphism in studied material specimens. A P value of less than 0.05 was considered statistically significant. Significant probability values obtained were analyzed for multiple testing using Bonferroni correction (P value after Bonferroni correction (P corr)). Post hoc power analysis with preestablished effect size, error probability, and sample size was performed using the G*Power version 3.1.3 program []. Statistical analysis was performed using STATISTICA 6.0 software (Statsoft, Tulsa, OK, USA). […]

Pipeline specifications

Software tools G*Power, Statistica
Application Miscellaneous
Diseases Glaucoma, Optic Nerve Diseases