Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: Sep 2 2017
Last update date: Nov 20 2018
Access: Public
Chemicals: Tamoxifen
Genes: HOXA5
Computational protocol: BCIP, TopHat, Cufflinks, DAVID, Primer-BLAST, GEO, Allen Brain Atlas
Dataset link Transcriptomic analysis of brainstem samples from mutants with Hoxa5 postnatal inactivation versus controls at postnatal day 21

Experimental Protocol


To explore HOXA5 downstream target genes in the postnatal brainstem, we carried out transcriptomic analyses by RNA-Seq using a model of postnatal Hoxa5 loss-of-function. We induced Hoxa5 inactivation after birth (P1 to P4) using the tamoxifen-inducible CMV-CreERT2 mice and conditional Hoxa5 floxed allele mice (Hoxa5flox). RNA was extracted from the brainstem of P21 tamoxifen-treated Hoxa5flox/flox;CMV-CreERT2+/- pups and from tamoxifen-treated Hoxa5flox/flox;CMV-CreERT2-/- littermates (see extract protocol).

Repositories


GEO

GSE103390

ENA

SRP131338

BioProject

PRJNA401713

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Contact


Françoise Gofflot
Françoise Gofflot

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