|Application:||Gene expression microarray analysis|
|Number of samples:||18|
|Release date:||Mar 11 2011|
|Last update date:||Jul 26 2018|
|Dataset link||Attenuation of EIF4E Translation Initiation Adversely Affects Multiple Myeloma Cells|
Multiple Myeloma cell lines RPMI 8226 and CAG were each transiently transfected with purified plasmids of tetraspanins: pEGFP-N1 (N1, control), CD81N1-eGFP (81N1) or CD82N1-eGFP (82N1) (Clontech). Transfected cells were separated by Sorter Flow Cytometer 24 hours after transfection. Total RNA was extracted from sorted transfected cells with the Qiagen kit. Three separate experiments were analyzed by Whole Genome Affymetrix microarray chips (N1, 81N1, 82N1 in each cell line).