Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Mar 11 2011
Last update date: Jul 26 2018
Access: Public
Diseases: Multiple Myeloma
Dataset link Attenuation of EIF4E Translation Initiation Adversely Affects Multiple Myeloma Cells

Experimental Protocol


Multiple Myeloma cell lines RPMI 8226 and CAG were each transiently transfected with purified plasmids of tetraspanins: pEGFP-N1 (N1, control), CD81N1-eGFP (81N1) or CD82N1-eGFP (82N1) (Clontech). Transfected cells were separated by Sorter Flow Cytometer 24 hours after transfection. Total RNA was extracted from sorted transfected cells with the Qiagen kit. Three separate experiments were analyzed by Whole Genome Affymetrix microarray chips (N1, 81N1, 82N1 in each cell line).

Repositories


GEO

GSE27897

ArrayExpress

E-GEOD-27897

BioProject

PRJNA138205

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Contact


Metsada Pasmanik-Chor