Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Jul 30 2007
Last update date: Mar 17 2012
Access: Public
Dataset link Identification of differentially-expressed genes between FoxH1 morpholino and control in zebrafish embryos.

Experimental Protocol


34k expression arrays were generated by printing Oligo nucleotides from three different sets (MWG, Compugen and Operon) onto epoxy slides. Total RNA from 50 FoxH1 MO- and 50 control MO-injected embryos was amplified using an Ambion kit (Cat#1753), labeled with Cy dyes and hybridized overnight to the oligo chip. This procedure was performed three times independently, including one dye swap, and cRNA from FoxH1 morphants and control embryos were co-hybridized for a total of three hybridizations.

Repositories


GEO

GSE8076

BioProject

PRJNA100913

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Contact


Christopher Pan