Computational protocol: Lasiodiplodia sp. ME4-2, an endophytic fungus from the floral parts of Viscum coloratum, produces indole-3-carboxylic acid and other aromatic metabolites

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Protocol publication

[…] The ITS sequence of the endophytic fungus was analysed along with closely related sequences retrieved from GenBank using BLAST []. Sequences were aligned with Clustal W [] and manually edited. A phylogenetic tree was constructed using the neighbour-joining method [] with MEGA version 4.0 []. Tree topologies were evaluated using bootstrap analysis based on 1000 resamplings. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Chemicals Phenylethyl Alcohol