Computational protocol: Yearly fluctuations of flower landscape in a Mediterranean scrubland: Consequences for floral resource availability

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Protocol publication

[…] To find out whether inter-annual flowering pattern variability was constrained by phylogeny, we conducted tests of phylogenetic signal for continuous variables based on Bloomberg’s K assuming Brownian motion character evolution []. We first built a phylogenetic tree of the 23 species with phylomatic V.3 []. Polytomies were resolved manually using timetree databases []. Then, we used the phylosig function of phytools R package [] to detect phylogenetic signal in flowering density CV, flowering peak SD, and flowering duration SD. To estimate the significance of the observed phylogenetic signals, K values obtained with the real data were compared to K values obtained from randomizations (1000 trees in which species where shuffled across the tips of the phylogenetic tree) []. We found that variability in flower density, flowering peak and flowering duration was not constrained by phylogeny (). That is, phylogenetically related species did not show similar propensity to variation (or lack thereof) in flowering patterns. Therefore, phylogeny was not accounted for in subsequent analyses. […]

Pipeline specifications

Software tools PHYSIG, Phylomatic, Phytools
Databases TimeTree
Application Phylogenetics