Computational protocol: Genome Sequence of the Multiple-Protease-Producing Strain Geobacillus thermoleovorans N7, a Thermophilic Bacterium Isolated from Paniphala Hot Spring, West Bengal, India

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[…] Geobacillus thermoleovorans strain N7 (MCC 3175) is a rod-shaped, Gram-positive, aerobic, thermophilic bacterium, isolated from Paniphala Hot Spring (63 ± 1°C, pH 7.6 ± 0.2), located at Barabani, Asansol, West Bengal (23°45′33″N, 86°58′54″E). This strain belongs to the family Bacillaceae under the phylum Firmicutes. The strain grew on a nutrient agar plate at (60 ± 1ºC) with an incubation period of 16 to 20 h.The purified genomic DNA of N7 was sequenced on an Ion PGM sequencer () using a 318 chip. Raw reads totaling 14,977,65 bp were assembled using SPAdes version 3.8.0 into 226 contigs with a coverage of 52%. A genome comprising 3,400,891 bp was obtained, with the largest contig size being 1,88,606 bp and the smallest being 200 bp, with a GC content of 52.4%. The genome was annotated and analyzed using NCBI PGAP (, RAST server version 2.0 (), and PATRIC (). NCBI PGAP predicted 3,530 genes, with 3,403 coding sequences, 36 rRNA genes, and 86 tRNA genes with 5 noncoding RNA genes. Genes encoding for rhamnogalacturonan acetylesterase (EC, an enzyme responsible for deacetylation of the hairy region of pectin (), and xylose isomerase (EC, which is capable of xylose and glucose isomerization, have been found. Assimilatory nitrate reductase (EC and nitrite reductase (EC convert nitrate to nitrite and finally to ammonia. Three kinds of protease, i.e., serine protease, metalloprotease, and neutral protease, are also present in N7. The genome sequence of G. thermoleovorans CCB_US3_UF5, closest member of N7 (99% identity) does not have rhamnogalacturonan acetylesterase and neutral protease (). The bacterial neutral proteases (pH around 7) are very much in use in food industry because they generate less bitterness in hydrolyzed food proteins in comparison to animal proteases (). Wet lab experiments have already been done for the confirmation of the enzymatic activity of these industrially important enzymes. Apart from that, several genes of medically useful enzymes have been found, namely, l-asparaginase, glutaminase, and RNase P. L-asparaginase and glutaminase have anticancer activity and are used to treat acute lymphoblastic leukemia (). As reported, an engineered RNase P ribozyme variant has been used as a potential antiviral agent in reducing human cytomegalovirus gene expression and growth (). Xenobiotic-degrading genes encoding for enzymes like catechol-2,3-dioxygenase (EC and nitrilotriacetate monooxygenase (EC, which are responsible for the degradation of catechol and nitrilotriacetate, have been found. Genes for fructose and mannose metabolism, maltose and maltodextrin utilization, and fatty acid biosynthesis and degradation have also been found.Genes with multiple resistance to some heavy metals like cobalt, cadmium, chromium, and mercury and antimicrobials like polymyxin and melittin, have been found, which were present in strain N7 but not in its closest neighbor G. thermoleovorans CCB_US3_UF5 (). Genes for the uptake and biosynthesis of some compatible solutes like glycine, myo-inositol, l-proline, sarcosine, and trehalose were also found. Enzymes of the central metabolic pathways (i.e., glycolysis, TCA cycle, pentose phosphate pathway, glyoxylate cycle, and oxidative phosphorylation) were also found, which indicates the bacterium’s aerobic mode of respiration. […]

Pipeline specifications

Software tools PGAP, RAST
Application Genome annotation
Organisms Geobacillus thermoleovorans