Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jan 23 2008
Last update date: Aug 28 2018
Access: Public
Diseases: Neoplasms, Second Primary
Genes: CD8A, IL2, GZMB
Dataset link Functional and Genomic Profiling of Effector CD8 T Cell Subsets

Experimental Protocol


An important question in memory development is understanding the differences between effector CD8 T cells that die versus effector cells that survive and give rise to memory cells. In this study we provide a comprehensive phenotypic, functional and genomic profiling of terminal effectors and memory precursors, towards better understanding the generation of these subsets. The two effector subsets were FACS purified during the early expansion phase (Days 4-5 post-infection) and extensively analyzed for their phenotypic (eg. CD127, CD62L, CD27, Bcl-2, Granzyme B, etc.) and functional properties (cytokine production, cytotoxicity, homeostatic proliferation, recall proliferation), and gene expression profiles (by genome-wide microarray analyses). Mechanistic studies involving the extent of proliferation and duration of antigen stimulation on memory differentiation potential of effectors were also performed using adoptive transfer techniques.

Repositories


GEO

GSE10239

ArrayExpress

E-GEOD-10239

BioProject

PRJNA108417

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