Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Feb 8 2018
Last update date: Jun 11 2018
Access: Public
Diseases: Tumor Lysis Syndrome
Dataset link Gene expression profiling of GSK3 inhibitor (CHIR99021)-treated intestinal organoids

Experimental Protocol


For microarray analysis, we performed two independent series of experiments. Total RNA was extracted from intestinal organoids cultured in the ENR media supplemented with or without CHIR99021 (5 microM) for four days as described above. Synthesis of cDNA, in vitro transcription and labelling of cRNA, and hybridization to the Mouse Gene 1.0 ST arrays (Affymetrix, Santa Clara, CA, USA) were performed according to the manufactures’ protocols. The CEL files were analyzed by Transcriptome Analysis Console (TAC) 4.0 software (Thermo Fisher Scientific). Expression signals of all genes (probe sets) were calculated using the RMA algorithm.

Repositories


GEO

GSE110257

BioProject

PRJNA433318

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