|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||12|
|Release date:||Jun 8 2009|
|Last update date:||Jun 11 2013|
|Dataset link||Endogenous small RNAs of meristematic and a terminally differentiated tissue of rice.|
Total RNA was extracted from root apices, shoot apices, developing inflorescence and mature leaf. Three samples from each tissue were taken to represent replicates and small RNAs from these were ligated to a common 5' RNA adapter and a replicate-specific 3' adapter. These replicate samples were then identified from within single 454 data sets using the 3' adapter sequence. It should be noted that there was a strong frequency bias observed between the RNA sequence and the particular 3' adapter used in the sequencing. This resulted in both increased or decreased read counts for the known miRNA sequences in a 3' adapter dependent fashion. Use of the three samples for each tissue as replicates is therefore problematic.