Computational protocol: The neuromuscular system of Pycnophyes kielensis (Kinorhyncha: Allomalorhagida) investigated by confocal laser scanning microscopy

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Protocol publication

[…] Figure plates were assembled using Creative Suite 6 (Adobe, San Jose, California, USA). To obtain fine detail micrographs of entire specimens, maximum or average projections of three confocal scans done on the same day of the same specimen (one anterior, one central, and one posterior), using identical settings on the microscope, were merged using the Photoshop ‘photomerge’ feature with ‘auto’ settings.Transverse projections were done with ImageJ (IJ 1.46r) []. 3D reconstructions were done with Imaris 5.7.2 (Bitplane, Belfast, UK). […]

Pipeline specifications

Software tools ImageJ, Imaris
Application Microscopic phenotype analysis
Diseases Xerostomia