Computational protocol: Draft Genome Sequence of Jiangella alkaliphila KCTC 19222T, Isolated from Cave Soil in Jeju, Republic of Korea

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[…] Jiangella alkaliphila, which belongs to the genus Jiangella, is an aerobic, Gram-positive, halotolerant filamentous actinomycete with a high G+C content (). The genus Jiangella was first identified by Song et al. in 2005 (), including four haloduric species currently on the List of Prokaryotic Names with Standing in Nomenclature (). This taxon originally belonged to the family Nocardioidaceae within the suborder Propionibacterineae () and was finally placed in the family Jiangellaceae of the order Jiangellales under the class Actinobacteria (). However, its position in the phylogenetic tree is still unstable in the actinobacterial areas using 16S rRNA information. We sequenced the genome of the type strain Jiangella alkaliphila KCTC 19222T while considering that members of this group have been isolated from different environments (, , , ). This genome was compared with the previously sequenced Jiangella gansuensis DSM 44835T, which will provide further insight into the adaptation of these strains to different habitats and a better understanding of the school of “evolutionary taxonomy” ().Whole-genome sequencing of Jiangella alkaliphila strain KCTC 19222T was completed using a paired-end sequencing method with the Illumina HiSeq 2000 platform (Illumina, San Diego, CA, USA); 1.76 Gb of data were produced, and 8,488,890 reads were used to assemble the daft genome by the SOAPdenovo2 package (). The draft genome size of J. alkaliphila is 7,567,836 bp in 124 scaffolds, with an average length of 61,031 bp and an N50 scaffold length of 169,179 bp. Its G+C content is 72.18% according to the genome. Genes were predicted using Glimmer version 3.02 (). The tRNAs and rRNAs were identified by tRNAscan-SE version 1.23 () and RNAmmer version 1.2 (). About 7,238 protein-coding genes, 47 tRNAs, and 4 rRNAs were predicted from this assembly. The predicted coding sequences (CDSs) were translated and used to search against the KEGG and COG databases. These data sources were combined to assert a product description for each predicted protein.The average nucleotide identity (ANI) was calculated between the genome sequences of J. alkaliphila KCTC 19222T and J. gansuensis DSM 44835T using the JSpecies software ( () based on the BLAST method (ANIb) and the MUMer algorithm (ANIm). The result shows that the ANI values between J. alkaliphila and J. gansuensis were below 85.3%, which confirmed that J. alkaliphila was a new species belonging to the genus Jiangella, since ANI values of 95 to 96% correspond to the 70% DDH cutoff point recommended by Wayne et al. () for the delineation of bacterial species (). […]

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