Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Oct 5 2012
Last update date: Feb 26 2016
Access: Public
Taxon: Vitis vinifera, Grapevine rupestris stem pitting-associated virus
Dataset link Co-evolution between Grapevine rupestris stem pitting-associated virus and Vitis vinifera L. induces a decrease in defence responses and physiological performance associated with an increase in photosynthesis-related gene transcription

Experimental Protocol


The study was carried out in a vineyard planted in 2002 in Albenga (Liguria), North-West Italy, where a row was established with the white grape cultivar Bosco (V. vinifera L.). Microarray analysis was carried out on leaves, petioles, and berries collected at véraison (E-L35) in 2010. For each of the six GRSPaV-free and six GRSPaV-infected vines selected for the physiological and agronomical parameters evaluation, we collected 6 leaves (3 basal and 3 apical) with the related petioles and 12 berries from 3 different bunches. Samples from each organ were arbitrary pooled in 3 independent biological replicates and total RNA was extracted according to the method described by Gambino et al. (2008).

Repositories


GEO

GSE36632

ArrayExpress

E-GEOD-36632

BioProject

PRJNA153629

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Contact


Marianna Fasoli