Dataset features


Application: Gene expression microarray analysis
Number of samples: 13
Release date: Aug 25 2009
Last update date: Mar 21 2012
Access: Public
Diseases: Alzheimer Disease, Neoplasms, Adipose Tissue, Intervertebral Disc Degeneration
Dataset link Gene Expression Data from Human Mesenchymal Stem Cells with and without TGF-B vs Human Annulus Disc Cells

Experimental Protocol

AD-MSC were extracted from human adipose tissue, and characterized as stem cells using accepted criteria (direction into osteoblasts or chondrocytes; and cell surface marker criteria). Three AD-MSC cultures were grown in 3D with or without TGF-ß3 for 2-3 weeks. Disc Tissue samples were obtained from surgical disc procedures performed on patients with herniated discs and degenerative disc disease. Seven disc cultures were also grown in 3-D for 2 weeks. RNA was harvested according to instructions with the Trizol isolation method, checked for quality using the 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA USA), reverse-transcribed to double-stranded cDNA, subjected to two rounds of transcription, and hybridized to the DNA microarray in the Affymetrix Fluidics Station 400. Affymetrix human U133 X3P arrays were used. Using Genesifter, gene expression in AD-MSC with and witout TGF-B3 was compared to annulus cells. Gene expression of stem cells with TGF-ß3 was also compared to stem cells grown in the absence of TGF-ß3.










Helen Gruber