Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 67
Release date: Apr 25 2016
Last update date: Sep 27 2017
Access: Public
Diseases: Parkinson Disease, Neurodegenerative Diseases
Dataset link Expression data from human patients with slow or rapid Parkinson's Disease progression

Experimental Protocol


Based on distinct disease stages and rates of motor disease progression, 70 Parkinson's Disease patients were clinically evaluated and divided into the two groups: slow (n=35) or rapid (n=35) progression. RNA was extracted from whole blood samples of these patients. RNA quality was assessed using RIN in the two groups of patients, which displayed mean RIN values of 7.14 (rapid progressors) and 7.02 (slow progressors). From the 70 RNA samples, 3 had RIN<6 and were not included in subsequent analysis (this corresponded to 1 rapid and 2 slow progressors samples). Expression analysis included 67 array samples (34 rapid and 33 slow progressors).. Gene expression profiling was performed using RNA expression data generated by genome array plates (Affymetrix Human Genome U219 platform, HG-U219). Patients Classification: Patients Classification: Patients were consecutively recruited from the movement disorders outpatient clinic of the Lisbon University Hospital. Neurologists with expertise in movement disorders interviewed and examined all cases. Patients were divided into two distinct groups with “Slow” or “Fast” progression, depending on the absence or presence of postural instability, respectively. Postural instability was defined by item 3.12 of the MDS-UPDRS, part III. Slow progression if scored 0 (no postural instability) and rapid progression if scored ≥ 1. Sample Collection: Venous blood from Parkinson's Disease patients was collected in PAXgene tubes

Repositories


GEO

GSE80599

BioProject

PRJNA319440

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Contact


Raquel Pinho