Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Aug 1 2013
Last update date: Aug 23 2018
Access: Public
Taxon: Homo sapiens, Mus musculus
Dataset link Location of indigenous human HSCs reveals functional properties of HSCs that are dictated by anatomic and cellular architecture of bone marrow II

Experimental Protocol


Total bone marrow cells were collected from NOD/SCID recipient adult mice. Osteoblast cells (OB) were sorted based on five paramater markers combination Lin- CD45- CD31- Sca1- CD51+ using a FACSAria II (BD). Total RNA from purified populations was extracted and amplified as described previously (Shojaei et al., 2005). Amplified-labeled RNA was hybridized to HG-U133Plus v2.0 chip.

Repositories


GEO

GSE47935

ArrayExpress

E-GEOD-47935

BioProject

PRJNA208388

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Contact


Mickie Bhatia