|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Aug 1 2013|
|Last update date:||Aug 23 2018|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Location of indigenous human HSCs reveals functional properties of HSCs that are dictated by anatomic and cellular architecture of bone marrow II|
Total bone marrow cells were collected from NOD/SCID recipient adult mice. Osteoblast cells (OB) were sorted based on five paramater markers combination Lin- CD45- CD31- Sca1- CD51+ using a FACSAria II (BD). Total RNA from purified populations was extracted and amplified as described previously (Shojaei et al., 2005). Amplified-labeled RNA was hybridized to HG-U133Plus v2.0 chip.