|Application:||Gene expression microarray analysis|
|Number of samples:||20|
|Release date:||Jun 10 2011|
|Last update date:||Mar 23 2012|
|Diseases:||Creutzfeldt-Jakob Syndrome, Encephalopathy, Bovine Spongiform|
|Dataset link||Bovine spongiform encephalopathy infection alters endogenous retrovirus expression in distinct brain regions of cynomolgus macaques (Macaca fascicularis)|
Cynomolgus macaques (Macaca fasicularis) were infected intracerebrally with BSE-positive brain stem material from cattle and allowed to develop prion disease. Brain tissue from the basis pontis and vermis cerebelli of the six animals and the same regions from four healthy controls were subjected to ERV expression profiling using a retrovirus-specific microarray and quantitative real-time PCR. In a second approach, ERV-K-(HML-2) RNA and protein expression was analysed in extracts from the same cynomolgus macaques.