
Similar protocols
Protocol publication
[…] Sequences of PHO proteins were retrieved from GenBank. Arabidopsis PHO proteins were used as queries in BLAST searches against the L. japonicus genome database. Conserved motifs in PHO proteins were analyzed using the NCBI’s CDD database (). The plastid transit peptide cleavage site of the PHO proteins was predicted using the program TargetP Server v1.01 (). For phylogenetic analysis, multiple sequence alignments of PHO amino acid sequences were performed using ClustalW. The tree was constructed using the neighbor-joining (NJ) method and 100 bootstraps in order to group putative full-length PHO amino acid sequences, and the results were displayed with Mega software version 4 (). […]
Pipeline specifications
Software tools | TargetP, Clustal W, MEGA |
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Applications | Phylogenetics, Protein sequence analysis |
Organisms | Lotus japonicus, Branchiostegus japonicus |