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Cell counting is an indispensable and essential problem because it directly affects the efficiency of many cell-based gene expression systems like Baculovirus Expression Vector System (BVES). Traditionally, this task is usually performed by microscopic-based counting. For example, the Neubauer, Burker and Fuchs-Rosenthal chambers are well known methods for counting cells in different cell concentration of interest. However, all of these methods have to be manually manipulated and therefore are prone to cause errors for the same person or different persons. Furthermore, most of them require frequent repetitions for validations.
(Sui., 2014) Bright field microscopic cells counting method for BEVS using nonlinear convergence index sliding band filter. Biomed Eng Online.