As for the cell tracking, software solutions have been adapted or developted for tracing cell lineage from phase contrast microscopy. In contrast to fluorescence microscopy technologies, phase contrast causes no phototoxicity dammage and has no need to dyeing cell samples. Phase contrast microscopy brings a non-fluorescent imaging technique with a great constrast allowing cell lineage tracing experiments.
A software tool for lineage editing and validation. LEVER segments, tracks and lineages time lapse microscopy image sequences of live stem cells. LEVER works in 2-D and 3-D and with phase and fluorescence images. LEVER is being used with adult and embryonic mouse neural stem cells, hematopoietic stem cells and also with human lung cancer and neuroblastoma cells.
Serves for time-lapse images of biological cells, colonies, and particles. LM is a program useful for detecting 2D dynamic single cell behaviors: migration, mitosis, cell death, cells within sheets, and cells moving with high cell-cell contact. This tool includes three mains skills: (1) operates on segmented masks; (2) uses biological properties measured from the segmented images to detect mitosis; and (3) uses the overlap information between current and past frames to identify and separate cells mistakenly segmented.
An integrated graphical user interface written in MATLAB that implements methods for automating cell segmentation/lineage reconstruction. The automated analysis iteratively combines a set of extended morphological methods for segmentation, and uses a neighborhood-based scoring method for frame-to-frame lineage linking.
Provides an image analysis software for prokaryotes with complex morphologies. BacStalk is an application specialized for automated, label-free, time-resolved image analysis of stalked and non-stalked bacteria. It enables the detection of fluorescence patterns and the morphometric analysis of cell bodies, and their corresponding stalks and buds. It can also visualize fluorescence signals in cells in interactive demographs and kymographs.
Discover our proposed protocols. They are easy to use or edit to meet your needs.