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Cell tracking software tools | Phase contrast microscopy image analysis

For instance, the nuclei of live cells are stained with a fluorescent dye and the cells are tracked using fluorescent imaging, although this type of approach has the potential disadvantage that excitation of fluorescent dyes in cells causes phototoxicity, hindering accurate characterization of cells. In another approach, non-fluorescent imaging, for example, phase contrast or differential interference contrast (DIC) imaging, is used to visualize cells and live cell movies are used for cell tracking , although the approach has never been used to analyze large numbers of cells.

Source text:
(Sato., 2016) Single-cell lineage tracking analysis reveals that an established cell line comprises putative cancer stem cells and their heterogeneous progeny. Sci Rep

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