For instance, the nuclei of live cells are stained with a fluorescent dye and the cells are tracked using fluorescent imaging, although this type of approach has the potential disadvantage that excitation of fluorescent dyes in cells causes phototoxicity, hindering accurate characterization of cells. In another approach, non-fluorescent imaging, for example, phase contrast or differential interference contrast (DIC) imaging, is used to visualize cells and live cell movies are used for cell tracking , although the approach has never been used to analyze large numbers of cells.
A user-friendly opensource software tool for tracking cells imaged with various imaging modalities, including fluorescent, phase contrast, and differential interference contrast (DIC) techniques. The main goals of the software tool are: (i) automated image quality enhancement using vignetting and alignment correction, (ii) detection and tracking of cells, (iii) editing cell paths and statistical analysis of the cell motion.
Segments and tracks budding yeast cells. DISCO is a comprehensive framework structured into several stages for integrated identification, segmentation and tracking of cells: (i) identification of physical features of the microfluidic device, (ii) supervised classification to identify cell centers, (iii) segmentation using a morphologically constrained cell-shape model, (iv) incorporation of temporal information to refine cell center prediction and (v) iterative greedy optimization of cell contours.
Enables cell lineage tracking. MicrobeTracker utilizes cell shape and timelapse information to achieve cell outlining. It can track fluorescently labeled molecules in cell lineages over several generations or in difficult-to-resolve samples, such as densely-packed or filamentous cells, from time-lapse sequences. This tool is delivered with an accessory tool, called SpotFinder, that detects small round spots, generating precise cell coordinates of fluorescently labeled foci inside cells.
You can access more results by creating a free plan account or unlimited content via a premium account.