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DISCO / Data Informed Segmentation of Cell Objects
Segments and tracks budding yeast cells. DISCO is a comprehensive framework structured into several stages for integrated identification, segmentation and tracking of cells: (i) identification of physical features of the microfluidic device, (ii) supervised classification to identify cell centers, (iii) segmentation using a morphologically constrained cell-shape model, (iv) incorporation of temporal information to refine cell center prediction and (v) iterative greedy optimization of cell contours.
CellStar
Permits analysis of video microscopy data for long-term experiments. CellStar has been designed to achieve good performance and was compared with six software solutions dedicated to yeast cell segmentation and tracking in bright-field microscopy. It segments yeast cells using bright-field images and tracks their trajectories through time with excellent accuracy. The tool is easy to use and allows the manual correction of segmentation and tracking errors through easy point-and-click procedures.
CASA / Computer Assisted Sperm Analyzer
Allows analysis of fish sperm. CASA can generate reproducible and quantitative measurements for fish sperm. The software facilitates rapid assessment of percent motility, velocity and other descriptors. It has been employed for several tasks: in determination of sperm variability among males; cryo-preservant effectiveness; the effects of heavy metals, quantification of differences in duration and initial speed between sperm of bluegill adopting different reproductive tactics; and elucidation of the effect of various ions on path curvature of trout sperm.
MORE software analysis / morpho-rheological software analysis
Allows high-throughput single cell morpho-rheological (MORE) characterization of all major blood cells in continuous flow. MORE software analysis allows individual blood cell mechanics to be studied in a range of human diseases. Label-free, disease-specific MORE blood signatures are a novel resource for generating hypotheses about the underlying molecular mechanisms. In addition, MORE software analysis has the potential to become a standard approach in flow cytometry with many applications in biology, biophysics, biotechnology and medicine.
TLA / Time Lapse Analyser
A free, cross-platform, open source software for high throughput time-lapse data processing for live cell imaging. TLA is a graphical tool which enables easy access to high-throughput live cell imaging for every user, regardless of the individual expertise. Beginners can easily process stacks of time-lapse data by loading an appropriate image processing setup and time-lapse evaluation setup. Advanced users can modify existing setups, or create new setups which suit their need and special experimental conditions.
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