CellTracer protocols

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CellTracer specifications

Information


Unique identifier OMICS_05636
Name CellTracer
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages MATLAB
License GNU General Public License version 2.0
Computer skills Medium
Version 1.0.3
Stability Stable
Maintained Yes

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Maintainer


  • person_outline Quanli Wang <>

Publication for CellTracer

CellTracer in pipeline

2017
PMCID: 5379763
PMID: 28376782
DOI: 10.1186/s12918-017-0399-z

[…] to become an important discipline in bioinformatics and computer vision []. for bacterial image processing, well known open source software packages for analyzing cell movies are the tlm-tracker [], celltracer [], microbetracker [] and its successor oufti [], and schnitzcells []. the tlm-tracker [] uses multiple alternative algorithms for cell segmentation, such as threshold-based, watershed […]


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CellTracer in publications

 (13)
PMCID: 5940870
PMID: 29739946
DOI: 10.1038/s41467-018-04134-7

[…] across each of the three notch conditions, indicating that the sorting experiments were not affected by differences in cell viability., we also stained the purified d4 he before plating with celltracer to track cell proliferation. when we analyzed the cells in each of the three notch conditions on d4 + 1, we found that there was a statistically significant increase in the proportion […]

PMCID: 5673064
PMID: 29105654
DOI: 10.1038/s41467-017-01477-5

[…] rna miniprep kit (r2050, zymo research, irvine, ca)., to monitor cell division, 20 million splenic or bone marrow cells from wt or stat5 dki mice were labelled in 1 ml of pbs with 2.5 μm of cfse (celltracer cfse cell proliferation kit, invitrogen, carlsbad, ca) at room temperature for 7 min, washed once with serum and twice with complete rpmi-1640 medium, 1.5 × 106 ml−1 cfse-labelled cells […]

PMCID: 5586347
PMID: 28904858
DOI: 10.1002/2211-5463.12262

[…] phagocytosis and to allow visualization under the microscope, agrbc, igg‐opsonized rbc, and c3bi‐rbc were labeled with the vital dye carboxyfluorescein succinimidyl ester (cfse) according to the celltracer™ cfse cell proliferation kit instructions. with igg‐opsonized rbc, these particles were incubated with cfse prior to fixation with pfa., before internalization of the different rbc models, […]

PMCID: 5428974
PMID: 28453519
DOI: 10.1371/journal.pgen.1006746

[…] roche)., cd4+ naive t cells were isolated from spleen of c57bl/6 (wt) and arhgef2-/- mice by using cd4+cd62l+ t cell isolation kit (miltenyi biotec, san diego, ca, usa) and subsequently stained with celltracer violet cell proliferation kit (thermo fisher scientific) according to the instructions provided. cells were then incubated with 2 μg/ml of anti-cd28 antibody (bd, new jersey, usa) […]

PMCID: 5379763
PMID: 28376782
DOI: 10.1186/s12918-017-0399-z

[…] to become an important discipline in bioinformatics and computer vision []. for bacterial image processing, well known open source software packages for analyzing cell movies are the tlm-tracker [], celltracer [], microbetracker [] and its successor oufti [], and schnitzcells []. the tlm-tracker [] uses multiple alternative algorithms for cell segmentation, such as threshold-based, watershed […]


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CellTracer institution(s)
Department of Statistical Science, Duke University, Durham, NC, USA

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