Computational protocol: Crystal structure of tRNA m1A58 methyltransferase TrmI from Aquifex aeolicus in complex with S-adenosyl-l-methionine

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Protocol publication

[…] The phase was determined by the molecular replacement method, using the coordinates of TrmI from Thermotoga maritima (PDB ID: 1O54) as the starting model, with the program MOLREP []. The model was completed using iterative cycles of manual rebuilding in Coot [] and computational refinement at 2.2 Å in Refmac5 [] (Table ). […]

Pipeline specifications

Software tools Molrep, Coot, REFMAC5
Application Protein structure analysis
Organisms Thermus thermophilus, Pyrococcus abyssi, Aquifex aeolicus
Chemicals Adenine, Hydrogen, S-Adenosylmethionine