Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: May 15 2017
Last update date: Nov 29 2018
Access: Public
Genes: TFAM, PHB2
Taxon: Homo sapiens
Computational protocol: Bowtie, MAnorm
Dataset link RNA-seq transcriptonal profiling in human primary adult erythroid progenitor cells (ProEs) after shRNA-mediated depletion of TFAM and PHB2 expression

Experimental Protocol


Human primary adult erythroblasts were generated ex vivo from CD34+ hematopoietic stem/progenitor cells (HSPCs) using a serum-free two-phase liquid culture system. CD34+ HSPCs were transduced with lentiviruses containing shRNAs against TFAM, PHB2, or the non-targeting (shNT) control, selected and differentiated to proerythroblasts (ProEs). Cells were harvested at day 5 of erythroid differentiation and total RNA were extracted for RNA-seq analysis.

Repositories


GEO

GSE86910

ENA

SRP089927

BioProject

PRJNA343063

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Contact


Jian Xu

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