Computational protocol: Glutamate transporter splice variant expression in an enriched pyramidal cell population in schizophrenia

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Protocol publication

[…] All data sets were analyzed for normal distribution. Outliers >3 s.d. from the mean were excluded. Correlation analysis was performed to determine associations between transcript expression and age, PMI and RIN value. One-way analysis of covariance was performed if significant correlations were found. If no correlations were present, data were analyzed with Student's t-test. The significance level was P<0.05 for all the statistical tests. Secondary analyses were performed to assess the effects of sex and medication on the dependent measures. To test for possible medication effects, patients with schizophrenia off antipsychotic medication for at least 6 weeks before death were compared with patients on antipsychotic medication within 6 weeks of death in a post hoc analysis. All data sets were analyzed with Statistica (Statsoft, Tulsa, OK, USA).Rodent data were analyzed for normal distribution. Outliers >3 s.d. from the mean were excluded. Data were log transformed and analyzed by Student's t-test and the significance level was P<0.05 for all the statistical tests. Data were analyzed with Graphpad Prism v6.04 (GraphPad Software, San Diego, CA, USA). [...] Genotype frequency was observed by direct counting. Hardy–Weinberg equilibrium was examined for all polymorphisms using Haploview software. Mann–Whitney U-test was applied to examine log-transformed splice variant mRNA expression in subjects homozygous for the major allele and subjects who were carriers (homozygous and heterozygous) of the minor allele. Data were analyzed with Graphpad Prism v6.04 (GraphPad Software). […]

Pipeline specifications

Software tools Statistica, Haploview
Applications Miscellaneous, GWAS
Chemicals Amino Acids, Glutamic Acid