Dataset features

Specifications


Application: ChIP-seq analysis, RNA-seq analysis
Number of samples: 27
Release date: Dec 19 2013
Last update date: Nov 14 2017
Access: Public
Genes: PRC1, CBX6, CBX7, CBX8, RING1, RNF2
Taxon: Homo sapiens
Dataset link Genome wide co-localization of Polycomb orthologs and their effects on gene expression in human fibroblasts

Experimental Protocol


Chromatin immunoprecipitation and sequencing (ChIP-seq) in two strains of human fibroblasts using antibodies against three Pc orthologs (CBX6, CBX7 and CBX8) and both Psc orthologs (RING1 and 2). Validation by ChIP-PCR and re-ChIP. Strand specific RNA-seq and validation by qRT-PCR.

Repositories


GEO

GSE40740

ArrayExpress

E-GEOD-40740

ENA

SRP015678

BioProject

PRJNA174794

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Contact


Gordon Peters