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CFX Manager specifications


Unique identifier OMICS_29002
Name CFX Manager
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Registration required Yes
Maintained No


  • DeltaDeltaCt


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CFX Manager citations


A Mechanism of Unidirectional Transformation, Leading to Antibiotic Resistance, Occurs within Nasopharyngeal Pneumococcal Biofilm Consortia

PMCID: 5954218
PMID: 29764945
DOI: 10.1128/mBio.00561-18
call_split See protocol

[…] 1 × 10−3 pg of chromosomal DNA from the appropriate serotype were run in parallel to generate a standard curve. This standard curve was then used to calculate the eDNA concentration using the Bio-Rad CFX Manager software. […]


ICAM 1 related long non coding RNA: promoter analysis and expression in human retinal endothelial cells

BMC Res Notes
PMCID: 5944171
PMID: 29743093
DOI: 10.1186/s13104-018-3384-8

[…] d methodology, using iQ SYBRGreen Supermix (Bio-Rad) and on the CFX96 Connect Real-Time PCR Detection System (Bio-Rad) []. Relative expression was calculated in the Gene Expression Analysis module of CFX Manager v3.1 (Bio-Rad), which uses the 2−ΔΔCt method [], normalizing to two stable reference genes. Primer sequences are given in Additional file : Table S4. As presented in Fig. , we observed tha […]


Mobilization of retrotransposons as a cause of chromosomal diversification and rapid speciation: the case for the Antarctic teleost genus Trematomus

BMC Genomics
PMCID: 5941688
PMID: 29739320
DOI: 10.1186/s12864-018-4714-x

[…] ainst Ct values. We estimated copy numbers of DIRS1, Gypsy and Copia family relative to both single copy (RAG1 on the one hand, and Rhodopsin on the other hand) calibration measurements using Bio-Rad CFX Manager v3.1, by comparison of their respective C(t) values, normalized to the DNA concentration initially present in the sample dilution. The slope of the standard curves was calculated and the a […]


Rhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia ovis Infection: Identification of Candidate Protective Antigens

PMCID: 5945973
PMID: 29780749
DOI: 10.3389/fcimb.2018.00116

[…] ocol. The average expression stability (M-value) of the reference genes, β tubulin, β actin, elongation factor, and 16S, was assessed based in geNorm algorithm (Vandesompele et al., ) included in the CFX Manager™ Software (Bio-Rad, CA, USA) and gene relative quantification was evaluated using the CFX Manager™ Software including the Pfaff method (Pfaffl, ) using the above-mentioned reference genes […]


Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network

Sci Rep
PMCID: 5928089
PMID: 29712938
DOI: 10.1038/s41598-018-24935-6

[…] nal generated with SYBR Green I DNA dye was measured during the annealing steps. The specificity of the amplification was confirmed using a melting curve analysis. Data were collected and recorded by CFX Manager Software (Bio-Rad) and expressed as a function of the threshold cycle (CT). The relative quantity of the gene of interest was then normalized to the relative quantity of GAPDH (ΔΔCT). The […]


Genome wide identification of the auxin response factor gene family in Cicer arietinum

BMC Genomics
PMCID: 5921756
PMID: 29703137
DOI: 10.1186/s12864-018-4695-9

[…] s, 60 °C for 30 s). The specificity of the primer pairs was checked by melting-curve analysis performed by the PCR machine after 40 amplification cycles (60 to 95 °C). Fluorescence was analyzed using CFX Manager Software v2.1 (Bio-Rad). All amplification plots were analyzed using a base line threshold of 100 relative fluorescence units (RFU) to obtain Cq (quantification cycle) values for each gene […]

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CFX Manager institution(s)
Bio-Rad, Marnes-la-Coquette, France

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