CFX Manager statistics

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CFX Manager specifications

Information


Unique identifier OMICS_29002
Name CFX Manager
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Registration required Yes
Maintained No

Subtool


  • DeltaDeltaCt

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CFX Manager in pipelines

 (22)
2018
PMCID: 5897773
PMID: 29514850
DOI: 10.15252/embj.201797965

[…] performed in triplicates in 20 μl total volume in a bio‐rad cfx connect real‐time pcr detection system with gotaq qpcr master‐mix (promega, a6001). we used the c t threshold cycle determined by the cfx manager software in accordance with the 2−δδct method to analyze rt–qpcr data. the ratio relative to the untreated control sample is plotted., mrnaseq: adapters were trimmed from the 3′ ends […]

2017
PMCID: 5362637
PMID: 28386232
DOI: 10.3389/fphys.2017.00168

[…] in stable . actin (wang et al., ) was used to standardize transcript levels in each sample. the δδct method was used to normalize the relative expression of each gene using the software of bio-rad cfx manager v. 1.6.541.1028. the student's t-test (p < 0.05) was conducted for the evaluation of significance of mean values., compared with control (ck), cd did not inhibit the root and shoot […]

2017
PMCID: 5416772
PMID: 28053125
DOI: 10.1093/nar/gkw1329

[…] probes listed in . using the same primers and probes, samples were then analyzed in triplicate or quadruplicate and cq values for the samples and the internal reference (apob) were calculated using cfx manager software (bio-rad). the means of the cq values were used to calculate δcq values and these were then used to calculate transgene copy number using the \documentclass[12pt]{minimal} […]

2017
PMCID: 5652791
PMID: 29100399
DOI: 10.18632/oncotarget.20497

[…] (quanta biosciences #95048-100). itaq™ universal sybrr green fluorescent supermix (biorad #172-5121) was used to quantify mrna expression. rna polymerase ii was used as an internal control. bio-rad cfx manager software was used to perform relative quantification of target genes using the comparative ct (δδct) method. primers were obtained from integrated dna technologies (idt, coralville, iowa) […]

2017
PMCID: 5738168
PMID: 29258576
DOI: 10.1186/s13071-017-2569-7

[…] transcript expressions were evaluated by rt-qpcr using the gene-specific primers and amplification conditions described in (additional file : table s1). the expression levels were analyzed with cfx manager software version 3.1 (bio-rad) and normalized to the g. pallidipes housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (gapdh) (vectorbase accession number gpai033271). fold change […]


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CFX Manager in publications

 (1965)
PMCID: 5955915
PMID: 29769533
DOI: 10.1038/s41467-018-04272-y

[…] an excitation wavelength of 450–490 nm, the emission spectra were collected using a 560–580 nm range fluorescence resonance energy transfer (fret) filter. the raw data were initially analyzed by cfx manager (version 1.6) and the sigmoidal dependence of fluorescence as a function of temperature was fitted to boltzmann equation to calculate the tm directly from the inflection point […]

PMCID: 5954218
PMID: 29764945
DOI: 10.1128/mBio.00561-18

[…] × 10−3 pg of chromosomal dna from the appropriate serotype were run in parallel to generate a standard curve. this standard curve was then used to calculate the edna concentration using the bio-rad cfx manager software., serotype-specific pcrs were performed in 25-µl volumes containing ~100 ng dna or 3 µl of bacterial lysate, 1 µm serotype-specific forward or reverser primer listed in , and 1× […]

PMCID: 5945781
PMID: 29748549
DOI: 10.1038/s41598-018-25596-1

[…] 30 sec in the bio-rad cfx connect machine (bio-rad). viral rna quantity was calculated using a full-length cdna plasmid as the copy-number standard, normalized with actin mrna by using the bio-rad cfx manager v3.1 (bio-rad), and expressed as the copy number per milliliter or per gram., student’s two-tailed t-test and one-way anova were used to compare the differences between 2 and multiple […]

PMCID: 5940204
PMID: 29738579
DOI: 10.1371/journal.pone.0196977

[…] with 2 × 50 μl aliquots of buffer ae and stored at 4°c., baicd gene cluster qpcr was performed with a cfx 96™ real-time pcr detection system (bio-rad laboratories, hercules, ca, usa) and bio-rad cfx manager 3.1 software. baicd gene cluster-specific primers were used to target c. scindens and c. hiranonis with modified primers based on the publication by ou et al. []. these published baicd […]

PMCID: 5927443
PMID: 29708983
DOI: 10.1371/journal.pone.0196194

[…] the following conditions: one cycle of 95°c for 2 min; one cycle of 95°c for 5 s; one cycle of 60°c for 25 s; and 39 cycles of 95°c for 5 s and 60°c for 25 s. rt-qpcr data was analyzed using biorad cfx manager (3.0) software., treated and untreated wheat plants grown in fields in two consecutive years were harvested on 13 april 2011 and 15 april 2012, respectively. the spikes were threshed, […]


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CFX Manager institution(s)
Bio-Rad, Marnes-la-Coquette, France

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