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Chimera detection software tools | 16S ribosomal RNA sequencing data analysis

Once denoising and additional quality control processes are completed, chimeric sequences should be removed from the dataset. Chimeras are artificial recombinants between two or more parental sequences, and they are normally formed when prematurely…
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UCHIME
Desktop

UCHIME

A package that detects chimeric sequences from PCR with two or more segments,…

A package that detects chimeric sequences from PCR with two or more segments, avoiding to interpret them as novel species. UCHIME uses a database containing no chimera-sequences or detects chimeras…

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ChimeraSlayer
Desktop

ChimeraSlayer

A chimeric sequence detection utility, compatible with near-full length Sanger…

A chimeric sequence detection utility, compatible with near-full length Sanger sequences and shorter 454-FLX sequences (~500 bp).

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mothur
Desktop

mothur

Aims to be a comprehensive software package that allows users to use a single…

Aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. mothur builds upon previous tools to provide a flexible and…

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AmpliconNoise
Desktop

AmpliconNoise

AmpliconNoise is a collection of programs for the removal of noise from 454…

AmpliconNoise is a collection of programs for the removal of noise from 454 sequenced PCR amplicons. This project also includes the Perseus algorithm for chimera removal.

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WigeoN
Desktop

WigeoN

Examines the sequence conservation between a query and a trusted reference…

Examines the sequence conservation between a query and a trusted reference sequence, both in NAST alignment format. WigeoN is a flexible command-line based reimplementation of the Pintail algorithm.…

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CSP
Desktop

CSP

Detects chimeric fragments and improves single individual haplotyping (SIH)…

Detects chimeric fragments and improves single individual haplotyping (SIH) accuracy. CSP is based on that a chimeric fragment would correspond to an artificial recombinant haplotype and would differ…

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CATCh
Desktop

CATCh

Combines the advantages of different individual chimera detection tools. CATCh…

Combines the advantages of different individual chimera detection tools. CATCh produces more robust results when challenged with chimeric sequences having a low parent divergence, short length of the…

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DECIPHER
Desktop
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