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WigeoN

Examines the sequence conservation between a query and a trusted reference sequence, both in NAST alignment format. WigeoN is a flexible command-line based reimplementation of the Pintail algorithm. WigeoN is useful for flagging chimeras and anomalies only in near full-length 16S rRNA sequences. Although WigeoN provided effective chimeric sequence detection with full-length sequences, it lacked sensitivity at shorter sequence lengths. WigeoN is part of the Microbiome Utilities package.