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Chimera identification software tools | 16S ribosomal RNA sequencing data analysis

Once denoising and additional quality control processes are completed, chimeric sequences should be removed from the dataset. Chimeras are artificial recombinants between two or more parental sequences, and they are normally formed when prematurely terminated fragments reanneal to other template DNA during PCR amplification (Bradley and Hillis, 1997). Source text: Kim et al., 2013.

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