ChopNSpice specifications


Unique identifier OMICS_06898
Name ChopNSpice
Interface Web user interface
Restrictions to use None
License GNU General Public License version 2.0
Computer skills Basic
Stability Stable
Maintained Yes

ChopNSpice in publications

PMCID: 5417816
PMID: 28289178
DOI: 10.1074/mcp.M116.062125

[…] ion series within complex ms/ms spectra (). this kind of software was developed to interpret ms2 spectra of branched sumoylated peptides. in addition, hsiao et al. developed a database search tool (“chopnspice”) that can successfully allow the identification of sumo acceptor sites from endogenous sumoylated proteins (). nevertheless, the application of these two search strategies is not widely […]

PMCID: 3499415
PMID: 23166733
DOI: 10.1371/journal.pone.0049630

[…] extracted peptides were analyzed by lc-msms on an orbitrap velos (thermofisherscientific) exactly under the conditions described in hsiao et al. . data analysis was performed by the use of software “chopnspice” in combination with mascot as search engine. see supplementary material and methods in for details., for confocal laser-scanning microscopy (clsm) analysis, cos-1 were transfected using […]

PMCID: 3443746
PMID: 23060889
DOI: 10.3389/fpls.2012.00215

[…] for each target separately using often ms/ms data obtained from in vitro sumoylated proteins. such relatively simple ms/ms spectra are then analyzed with specific algorithms such as summon and chopnspice to facilitate annotation of both in vitro and biological data (pedrioli et al., ; hsiao et al., ; jeram et al., ). a second problem is that tryptic digestion of sumo leaves a large […]

PMCID: 3135405
PMID: 21746851
DOI: 10.1083/jcb.201010117

[…] the orbitrap detector, a lock-mass ion from ambient air (m/z = 445.120025) was used for internal calibration., identification of sumoylated lysine residues was performed exactly as described in . chopnspice software () was used to generate a concatenated protein sequence in order to identify the actual sumo sites with the mascot search engine. for this, the fasta sequence of α-synuclein […]

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