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Chromas specifications

Information


Unique identifier OMICS_01016
Name Chromas
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Version 2.6.4
Stability Stable
Maintained Yes

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Chromas citations

 (150)
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Isolation and characterization of Corynebacterium spp. from bulk tank raw cow's milk of different dairy farms in Germany

2018
PLoS One
PMCID: 5884509
PMID: 29617406
DOI: 10.1371/journal.pone.0194365
call_split See protocol

[…] m specific primers C2700F (5´-CGWATGAACATYGGBCAGGT-3´) and C3130R (5´-TCCATYTCRCCRAARCGCTG-3´) []. These primers were also used as sequencing primers. The obtained sequences were manually edited with Chromas Lite 2.1.1. (Technelysium Pty Ltd, South Brisbane, AU) and assembled with BioEdit 7.2.5. [] to obtain either almost complete 16S rRNA sequences of 1,400–1,500 base pairs (bp) or partial rpoB g […]

call_split

PCR RFLP assay as an option for primary HPV test

2018
Braz J Med Biol Res
PMCID: 5886552
PMID: 29590262
DOI: 10.1590/1414-431X20177098
call_split See protocol

[…] accuracy of the DNA sequencing was evaluated through the CAP3 program based on Phred quality score ().Sequences were aligned and compared to those available in the GenBank database using the software Chromas Lite 2.1 (Technelysium, Australia). HPV type was identified based on >90% sequence homology over 449–458 nucleotides. […]

library_books

A Comprehensive in Silico Analysis of Regulatory SNPs of Human CLEC7A Gene and Its Validation as Genotypic and Phenotypic Disease Marker in Recurrent Vulvovaginal Infections

2018
PMCID: 5869923
PMID: 29616193
DOI: 10.3389/fcimb.2018.00065

[…] PCR products obtained on gel was used to determine genotypes (Figure ). Verification of representative genotypes was done by Sanger sequencing (Figure ). These sequences were analyzed using the tool chromas 2.6.4 (http://technelysium.com.au/wp/chromas/). […]

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First report of Leishmania infantum infection in the endangered orangutan (Pongo pygmaeus pygmaeus) in Madrid, Spain

2018
PMCID: 5859647
PMID: 29554944
DOI: 10.1186/s13071-018-2772-1
call_split See protocol

[…] ard and reverse primers at the sequencing service of the Genomics department, UCM, using an ABI Prism 3730 system (Applied Biosystems, California, USA).Sequence chromatogram files were analysed using Chromas 2.1.1 and imported into BioEdit v.7.0.5 for editing, assembly and alignments. The sequences obtained were aligned with sequences available from GenBank using Clustal W and compared with additi […]

call_split

Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau

2018
PMCID: 5845295
PMID: 29523164
DOI: 10.1186/s13071-018-2684-0
call_split See protocol

[…] xy chain termination in an ABI 3730 DNA sequencer at Sangon Company (Shanghai, China). The PCR primers were used as sequencing primers. All the raw sequences were assembled using the software package Chromas, edited and blasted online (https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch) to determine the Echinococcus species or genotype of each cyst sample.A single complete mt genome wa […]

library_books

Molecular taxonomy and evolutionary relationships in the Oswaldoi Konderi complex (Anophelinae: Anopheles: Nyssorhynchus) from the Brazilian Amazon region

2018
PLoS One
PMCID: 5837296
PMID: 29505595
DOI: 10.1371/journal.pone.0193591

[…] The sequences of the COI and ITS2 markers were automatically aligned in ClustalW [] and edited in BioEdit v.7.2.5 [] with the aid of Chromas Lite []. The consensus sequences of the COI gene generated a fragment size of 663 base pairs (bp), which was translated into amino acids to check for stop codons, pseudogenes or Numts. These s […]

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Chromas institution(s)
Technelysium Pty Ltd, Brisbane, Australia

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