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BeStSel / Beta Structure Selection

A method for the secondary structure determination and fold recognition from protein circular dichroism spectra. BeStSel allows structural biologists to solve the secondary structure of their protein samples accurately and to gain more structural information. The versatile algorithm presents a significant improvement compared with existing ones. With fast CD spectral data acquisition and accurate structure determination, our algorithm provides biophysical scientists with a powerful tool.


Provides a means of testing protein circular dichroism (CD) spectral data and metadata for quality, completeness and consistency, as well as identifying unusual but potentially important deviations from standard spectral characteristics. One of the primary aims for creating the ValiDichro server was to increase good practice within the field of protein CD spectroscopy, especially providing users with guidance regarding data quality. This is important, as CD is a complementary technique often used in conjunction with other structural biology methods by those who are not experts in its application. ValiDichro can be used as a valuable guide for data collection, as well as a test for data quality before publication, and as an indication to users of the data of its validity.


The 2Struc server provides two functionalities; 2Struc, which generates secondary structure element (SSE) assignments for protein structures for up to eight different methods enabling easy analyses of similarities and differences between them, and Compare-the-Protein, which allows comparisons and highlighting of differences between the SSEs generated within a series of NMR models or between two x-ray structures. 2Struc is unique in providing summaries of percentage secondary structure content for reduced three-state data and original SSE output assignments where generated.

CAPITO / CD Anaylsis and Plotting Tool

A web server-based analysis tool for interpreting circular dichroism (CD) spectra. CAPITO allows the simultaneous evaluation of multiple datasets. Hence, it is suitable for the investigation of a protein in question under different conditions (temperature, pH, buffer solvent and mutations). Our approaches (basis spectra and matching-based method) to extract secondary structure information from a CD spectrum take advantage of a recent significant increase in the availability of well-calibrated far-UV CD spectra linked to available tertiary structures.


Predicts secondary structural content from a circular dichroism (CD) spectrum. K2D takes advantage of a recent notable development in the field of CD spectra estimation from structural data, DichroCalc, and of the high number of proteins in the Protein Data Bank (PDB), which covers a large range of structural configurations. When the size of the protein is included in the algorithm, K2D produces results comparable to other methods in terms of performance for the 190–240 nm interval and improves the performance for the 200–240 nm interval. K2D represents an improvement mostly in the predictions of beta-sheet content. Thus, we recommend using K2D if the size of the protein is known, especially if the CD spectrum is restricted to the 200 nm and up wavelength interval.