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Clotho specifications


Unique identifier OMICS_04723
Name Clotho
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


No version available


Unique identifier OMICS_04723
Name Clotho
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for Clotho

Clotho citations


Insulated transcriptional elements enable precise design of genetic circuits

Nat Commun
PMCID: 5495784
PMID: 28674389
DOI: 10.1038/s41467-017-00063-z

[…] he resolution of genetic circuit design to a new limit. Moreover, because our method works at a different layer, it is compatible with existing genetic circuit technologies including the “Cello” and “Clotho” software, , .In this work, synthetic transcriptional regulation was defined by specific interactions between defined components. For instance, transcriptional activation by T7 RNAP at the T7 p […]


Computational Tools and Algorithms for Designing Customized Synthetic Genes

Front Bioeng Biotechnol
PMCID: 4186344
PMID: 25340050
DOI: 10.3389/fbioe.2014.00041

[…] ological parts are immutable, and no optimizations are applied in the creation of the constructs. Other circuit design tools include GEC (Pedersen and Phillips, ), TinkerCell (Chandran et al., ), and Clotho (Xia et al., ).In Section “,” we examine some of the most important objectives in synthetic gene design toward optimized expression, accompanied by a brief analysis of their computational compl […]


Preparing synthetic biology for the world

Front Microbiol
PMCID: 3554958
PMID: 23355834
DOI: 10.3389/fmicb.2013.00005

[…] ing safeguards to help scientists prevent unintentional creation of dangerous organisms. These tools are intended to help scientists design safe synthetic systems before the system is actually built. CLOTHO is a design software tool that helps synthetic biologists construct and simulate engineered genetic devices (“Clotho,” last accessed October 22, 2012, A homology sea […]


Genome scale engineering for systems and synthetic biology

Mol Syst Biol
PMCID: 3564264
PMID: 23340847
DOI: 10.1038/msb.2012.66

[…] enerated across libraries of genomes. Traditional gene editors such as Vector NTI and SeqBuilder are largely inadequate for such purposes. However, new design tools and software suites such as J5 (), Clotho (), and Genome Compiler ( provide better data management and user interfaces for the design of large operons and whole genomes.Although recordkeeping is importan […]


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Clotho institution(s)
Department of Bioengineering, University of California, Berkeley, CA, USA

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