Coloc statistics

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Coloc specifications

Information


Unique identifier OMICS_25609
Name Coloc
Alternative name Colocalization analysis
Software type Application/Script
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Java
License GNU General Public License version 3.0
Computer skills Medium
Version 3.0.1
Stability Stable
Requirements
ImageJ
Source code URL https://codeload.github.com/fiji/Colocalisation_Analysis/zip/master
Maintained Yes

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Coloc in publications

 (295)
PMCID: 5958120
PMID: 29773788
DOI: 10.1038/s41467-018-04390-7

[…] with dapi (4',6-diamidino-2-phenylindole) (life technologies), dried and kept in the dark until examined by confocal microscopy (zeiss lsm 710 nlo). pcc for colocalization was calculated using the coloc2 plugin from imagej. at least ten representative images were analyzed to obtain the average coefficient., for tem of cells, particles were introduced to the raw 264.7 and j774a.1 cells […]

PMCID: 5943321
PMID: 29743474
DOI: 10.1038/s41467-018-04221-9

[…] access image analysis platform, fiji, using a macro to assign color to tmrm intensity ranges with maximum projections of mfi bile canaliculi images superimposed in 8-bit gray scale. the fiji plugin, coloc2, was used to generate mander’s correlations for above zero intensity of channel (m1, m2) and above auto threshold of channel (tm1, tm2) for whole images (×20, 0.4 na) and pearson’s […]

PMCID: 5943604
PMID: 29743632
DOI: 10.1038/s41598-018-25667-3

[…] statistical data for the co-localization levels, co-localization coefficients for green and red channels, overlap coefficient (r), pearson’s correlation coefficient (rr) was generated using the “coloc” option in the software. the number of co-localized pixels divided by the total number of green and red pixels was presented as the co-localization percentage., western blotting of hek293 cells […]

PMCID: 5943300
PMID: 29743550
DOI: 10.1038/s41419-018-0508-y

[…] were taken on a keyence bz9000 fluorescence microscope. hoechst was added 30 min prior to imaging. pearsons correlation coefficients were calculated using the fiji distribution of imagej and the coloc2 plugin., mefs were cultured on glass cover slips and fixed for 20 min in 4% pfa plus 1% glutaraldehyde (roth, germany) in pbs. after contrastation in 0.5% osmiumtetroxide (30 min at rt) cells […]

PMCID: 5943408
PMID: 29743604
DOI: 10.1038/s41467-018-03955-w

[…] a co-detected cdc42 spot (within −18 to +18 s time window) at the endocytic site, we identified two populations via manual classification (see s.i. for a detailed description). we called them cdc42 coloc (co-detection of cdc42 and secgfp-gpi) and cdc42 nocolc (the remainder). we compared the fold accumulation of all the cdc42 spots (cdc42 all), cdc42 coloc and cdc42 nocoloc with the random. […]


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